Sbi(https://www.systembio.com/)

System Biosciences (SBI) develops innovative research tools for Vectordelivery technologies, MicroRNA research, Exosome research, and GenomeEngineering tools, including the CRISPR-Cas9 SmartNuclease? system andPrecisionX? HR targeting vectors. Highlights: Vector Technologies: ? Powerfullentivector systems ? One-step transfection piggyBac transposons ? Single-copygene additions with phiC31 integrase vectors ? Sustained expression withoutintegration with Minicircle DNA plasmids Exosome Research Tools: ? Isolateexosomes with ExoQuick and ExoQuick-TC ? Exo-Flow- Immunopurify exosomes onmagentic beads and anlayze by FACS ? Culture cells in exosome-depleted FBS ?Verify exosome recovery with antibodies ? Quantitate exosomes with ELISAsGenome Engineering: ? RNA-guided Cas9 SmartNuclease system ? Fast and efficientmutation correction / gene editing ? HR donor vectors for knock-in, knock-out& tagging ? High-throughput, targeted integration with PinPoint technologyStem Cell Research: ? iPSC reprogramming systems ? Growth factors and media ?Pluripotency confirmation kits and antibodies ? iPS celllines, normal anddisease models ? Promoter lineage reporters in lentivectors Non-coding RNA: ?LncRNA qPCR profiling kits ? miRNome and OncoMir qPCR profiling systems ?Lenti-miR Precursor clone libraries – arrayed and pooled ? miRZip anti-miRclones for stable miRNA inhibition Exo-NGS Custom Service: ? Provide serum,tissue culture media, or other patient biofluids to SBI ? SBI isolatesexosomes, purifies RNA and generates high quality NGS libraries ? Sequencingperformed using Illumina MiSeq and HiSeq platforms ? Comprehensive dataanalysis, including generation of heat maps and top hits, provided ? NGS dataloaded onto a custom UCSC Genome Browser for convenient viewing.