Proteintech 60008-1-Ig，beta Actin Monoclonal Anbody;Proteintech.60008-1-Ig;Tested applicaons: ELISA, WB, IF, FC, IHC ;_Proteintech

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: 首页试剂蛋白杂交分析抗体 Proteintech 60008-1-Ig，beta Actin Monoclonal Anbody;Proteintech.60008-1-Ig;Tested applicaons: ELISA, WB, IF, FC, IHC ;

Proteintech 60008-1-Ig，beta Actin Monoclonal Anbody;Proteintech.60008-1-Ig;Tested applicaons: ELISA, WB, IF, FC, IHC ;

Proteintech.60008-1-Ig ; beta Actin Monoclonal Anbody ; Tested applicaons: ELISA, WB, IF, FC, IHC ;

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货品编号：Proteintech.60008-1-Ig

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品牌：Proteintech
所属分类：抗体

<p> </p>
<p>    Catalog number: 60008-1-Ig </p>
<p>    Size: 150 μg/150 μl </p>
<p>    Source: Mouse </p>
<p>    Isotype: IgM </p>
<p>    Synonyms:
ACTB; ACTB, Acn, acn, beta,
Acn, cytoplasmic 1, B acn,
Beta acn, F acn, β acn</p>
<p> </p>
<p>    Applications:</p>
    Tested applicaons: ELISA, WB, IF, FC, IHC <p>    Cited applicaons: IHC, WB </p>
<div>    Species specificity: Human, Mouse, Rat, Zebrafish, Pig, yeast; other
species not tested.
</div><div>    Cited species: Bovine, C. elegans, chicken, Drosophila, duck, fly,
human, mouse, pig, rat, rice, yeast, zebrafish
 </div><p>    Caculated beta Actin MW: 375aa,42 kDa
</p>
<p>    Observed beta Actin MW: 42 kDa
</p>
<div>    Posive WB detected in : Mul-cells/ssue, A549 cells, arabidopsis whole plant
ssue, HEK-293 cells, HeLa cells, MCF7 cells, whole
Nematode ssue, whole yeast cells
</div><p>    Posive IHC detected in: Human kidney ssue, human brain ssue, human
colon ssue, human heart ssue, human lung ssue,
human ovary ssue, human placenta ssue, human
spleen ssue, human tess ssue </p>
<p>    Posive IF detected in : HepG2 cells
</p>
<p>    Posive FC detected in: HepG2 cells
 </p>
<p>    Recommended diluon:    WB: 1:2000-1:20000
 </p>
<p>                                                  IHC: 1:20-1:200</p>
<p>                                                     IF: 1:20-1:200 </p>
<div>                                                   FC: N/A

</div><p> </p>
<p>    Background:</p>
<div>    Beta acn, also named as ACTB and F-Acn, belongs to the acn family. Acns are
highly conserved globular proteins that are involved in various types of cell molity
and are ubiquitously expressed in all eukaryoc cells. At least six isoforms of acns
are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1),
alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aorc smooth muscle), beta (ACTB),
gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two
non-muscle acn proteins. Most acns consist of 376aa, while ACTG2 (rich in
muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta acn
has been widely used as the internal control in RT-PCR and Western Blo0ng as a 42-
kDa protein. This anbody can recognize all the acns.

</div><p> </p>
<p>    Immunogen information:</p>
<p>    Immunogen: Ag0297 </p>
<p>    GenBank accession number: BC002409
</p>
<p>    Gene ID (NCBI): 60
</p>
<div>    Full name: Actin, beta

</div><p> </p>
<p>    Product information </p>
<p>    Purificaon method: Caprylic acid/ammonium sulfate precipitaon </p>
<p>    Storage: PBS with 0.1% sodium azide and 50% glycerol pH
7.3. Store at -20
oC.</p>
<p> </p>
<img src="http://solelybio.com/public/images/f3/8c/e4/31b5f5700f1353c68d6ce81647c26e08007c66f7.jpg?1445491709#w">
<p>Western blot analysis of Beta-acn in
various ssues and cell lines using
Proteintech anbody 60008-1-Ig at a
diluon of 1:5000. Extra bands were
detected in some species with
unknown reason.</p>
<p> </p>
<img src="http://solelybio.com/public/images/b0/5f/fc/eefc76ac0a11d79bbb171f81fba29ac800862f9d.jpg?1445495037#w">

<p>Western blot of Hela cell with an-
Acn-Beta (60008-1-Ig) at various
diluons.</p>
<p> </p>
<img src="http://solelybio.com/public/images/39/38/05/8a287fed0c0221637fc97784195c338739b3d95a.jpg?1445495037#w">

<p>Immunofluorescent analysis of
HepG2 cells using 60008-1-Ig( beta
acn Anbody) at diluon of 1:50
and N/A</p>
<p> </p>
<img src="http://solelybio.com/public/images/d1/f0/07/d5e5438a060d0582aee5c53c13099b44557d482c.jpg?1445495038#h">

<p>Immunohistochemical of
paraffin-embedded human
kidney using 60008-1-Ig(ACTB
anbody) at diluon of 1:100
(under 40x lens)</p>
<p> </p>
<img src="http://solelybio.com/public/images/49/48/d1/8a4c31d24c7884a2df01ff629cbc14acc6641344.jpg?1445495037#h">

<p>1X10^6 HepG2 cells were
stained with 0.2ug beta acn
anbody (60008-1-Ig, red) and
control anbody (blue). Fixed
with 90% MeOH blocked with
3% BSA (30 min). Alexa Fluor
488-congugated AffiniPure
Goat An-Mouse IgG(H+L)
with diluon 1:1000.</p>
<p> </p>

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