Proteintech 66008-2-Ig，Flagtag Monoclonal Anbody;Proteintech.66008-2-Ig; Tested applicaons: ELISA, WB, IP,

Proteintech.66008-2-Ig ;Flagtag Monoclonal Anbody; Tested applicaons: ELISA, WB, IP,

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货品编号：Proteintech.66008-2-Ig

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商品详情

品牌：Proteintech
所属分类：抗体

Catalog number: 66008-2-Ig 
    Size: 150 μg/150 μl 
    Source: Mouse 
    Isotype: IgG2b

    Synonyms:
DDK tag, DYKDDDDK, FLAG,
Flag tag
 
    Applications:
    Tested applicaons: ELISA, WB, IF, IP    Cited applicaons: 	 WB 
<div>    Species specificity: Human; other species not tested.

</div><div>    Cited species: Rat
</div>    Caculated Flag tag MW: -

    Observed Flag tag MW: -

<div>    Posive WB detected in :Recombinant protein, Transfected HEK-293 cells
</div>    Posive IP detected in : Recombinant protein
    Posive IF detected in: Transfected HEK-293 cells
    Recommended diluon:    WB: 1:5000-1:50000
 
                                                    IP:  N/A
                                                    IF: 1:20-1:200 
 
 
    Background:
<div>    Flag tag, also known as DDK tag, is a hydrophilic tag for recombinant protein
technology with the sequence DYKDDDDK. The structure of Flag tag has been
opmized for compability with the proteins it is aCached to and unlikely to
denature or inacvated them. For its high hydrophilic nature, the Flag tag is likely to
be located on the surface of a fusion protein, which enables the tag more likely to
be accessible to anbodies. This product is generated against 1xFlag tag
(DYKDDDDK) and can recognize protein containing one or more Flag tags, no
maCer it is/they are located at N-terminal, C-terminal or at internal regions. The
binding of this product with Flag tagged fusion protein does not depend on calcium
ion. The clone of this monoclonal anbody hybridoma is 1E7B4, which is different
from any other vendors.

</div> 
    Immunogen information:
    Immunogen: Pepde 
    GenBank accession number:  /
    Gene ID (NCBI):  /

<div>    Full name: Undefined

</div> 
    Product information 
    Purificaon method: Protein A purificaon

    Storage: PBS with 0.02% sodium azide and 50% glycerol pH
7.3. Store at -20
oC.

 
  
<img src="http://solelybio.com/public/images/2a/61/00/1257c56318743a5f5a0b2c6db3b475fb66656c91.jpg?1445496294#h">
    IP Result of an-Flag tag (IP:66008-2-
Ig, 3ug; Detecon:20543-1-AP 1:2000)
with Recombinant protein protein
lysate 1280ug. 
 
                 
<img src="http://solelybio.com/public/images/ec/0e/3d/28d7f51fb1d2d854066d75fb16705fa09353ee76.jpg?1445496294#h">
    Transfected HEK-293 cells were
subjected to SDS PAGE followed by
western blot with 66008-2-Ig( Flag tag
Anbody) at diluon of 1:1000

 
      
<img src="http://solelybio.com/public/images/2c/cd/c1/2d6d9e0f9a2a0c53669f5f7fe8a37f96dc35619c.jpg?1445496294#h">
     Immunofluorescent analysis of
Transfected HEK-293 cells using
66008-2-Ig( Flag tag Anbody) at
diluon of 1:50 and Alexa Fluor 594-
congugated AffiniPure Goat An-
Mouse IgG(H+L)
 
     
<img src="http://solelybio.com/public/images/ce/ce/b2/a0154dfb627c4bc70184fdd6b1bf8418ee88cac1.jpg?1445496294#h">
    Recombinant protein were subjected to SDS PAGE
followed by western blot with
66008-2-Ig (Flag tag Anbody)
at various diluons.