Proteintech 66002-1-Ig，GFP tag Antibody ; Proteintech.66002-1-Ig ; Tested applicaons: ELISA, WB, IP,IF

Proteintech.66002-1-Ig ;GFP tag Antibody; Tested applicaons: ELISA, WB, IP,IF

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货品编号：Proteintech.66002-1-Ig

数量： -+ 支

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商品详情

品牌：Proteintech
所属分类：抗体

Catalog number: 66002-1-Ig 
    Size: 213 μg/150 μl 
    Source: Mouse 
    Isotype: IgG2a
    Synonyms:  CFP, eGFP, eYFP, GFP, GFP tag,
YFP
 
    Applications:
    Tested applicaons: ELISA, WB, IF,IP    Cited applicaons:  IF, IP, WB 
<div>    Species specificity: Recombinant protein; other species not tested.

</div>    Cited species: Tag
    Caculated GFP tagn MW: 26 kDa

Observed GFP tag MW:  /
<div>    Posive WB detected in : Recombinant Protein
</div>    Posive IP detected in: Transfected HEK-293 cells

    Posive IF detected in : Transfected HEK-293 cells

Recommended diluon:    WB: 1:2000-1:20000  
                                                    IP: 1:1000-1:10000
                                                    IF: 1:10-1:100 
 
    Background:
<div>    Protein tags are protein or pepde sequences located either on the C- or Nterminal
of the target protein, which facilitates one or several of the following
characteriscs: solubility, detecon, purificaon, localizaon and expression. Green
fluorescence protein(GFP) is a protein composed of 238 amino acid
residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green
light(emission peak at 509nm) when excited by blue light(excitaon peak at 395nm).
GFP has become an invaluable tool in cell biology research, since its intrinsic
fluorescence can be visualized in living cells. EGFP contains the double-amino-acid
substuons Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1;
PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shiFed
excitaon peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP
when excited at 488nm, due to an increase in its exncon coefficient(Em). The
anbody recognizes the GFP-tag, eGFP tag, eYPF tag, CFP tag or YFP tag fused to
either the amino- or carboxy-terminus of targeted proteins in transfected
mammalian cells.

</div> 
    Immunogen information:
    Immunogen: Ag2128

    GenBank accession number: AVU73901

Gene ID (NCBI):  /
<div>    Full name: Undefined

</div> 
    Product information 
    Purificaon method: Protein A purificaon
    Storage: PBS with 0.02% sodium azide and 50% glycerol pH
7.3. Store at -20
oC
 
  
<img src="http://solelybio.com/public/images/17/4f/95/170b8b161460d0fb63acf23f4a00e37d860d870e.jpg?1445497228#h">
    WB result of eGFP fusion protein
with an-eGFP tag(66002-1-Ig) with
GFP fusion protein at varous
diluons.
 
      
<img src="http://solelybio.com/public/images/98/3e/e4/22e1c089b5c3a1f9d96d6a4dd6756f7538f702fa.jpg?1445497228#h">
    Immunofluorescent analysis of
Transfected HEK-293 cells using
66002-1-Ig( GFP tag Anbody) at
diluon of 1:25 and Alexa Fluor 488-
congugated AffiniPure Goat An-
Mouse IgG(H+L)

     
<img src="http://solelybio.com/public/images/cf/67/97/46f587ae636b81156c311e2776dfba2571888a6d.jpg?1445497228#h">
    IP Result of an-GFP tag (IP:66002-1-
Ig, 4ug; Detecon:66002-1-Ig 1:2000)
with Transfected HEK-293 cells lysate
300ug.