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ebioscience.17-0691-82 CD69 Monoclonal Antibody (H1.2F3), APC, eBioscience 100ug
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CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes
in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface
expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.
Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of stimulated mouse splenic cell cultures.
This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample
in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended
that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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