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ebioscience.11-0041-82 CD4 Monoclonal Antibody (GK1.5), FITC, eBioscience 100ug
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by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of
antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In
T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
Applications Reported: The GK1.5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes.
This can be used at less than or equal to 0.25 µg per test A test is defined as the amount (µg) of antibody that will stain a cell
sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It
is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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