TOYOBO .KMM-201 KOD ONE TM PCR Mix-Blue 1 ml ×5支

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货品编号：KMM-201

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品牌：Toyobo
所属分类：辅助货品

<p>     货品编号：  TOYOBO.KMM-201<span style="white-space: pre;">	</span></p>
<p>     品牌：  TOYOBO</p>
<p>     品名：  KOD ONE TM PCR Mix-Blue<span style="white-space: pre;">	</span></p>
<p>     规格：  1 ml ×5支</p>
<p> </p>
<p> </p>
<h3 id="d02" class="title odd first-child nth-child-1" style="margin: 0px; padding: 10px 10px 10px 20px; font-size: 1.9em; font-family: 黑体; height: 16px; background: url("../img/detail_title_bg02.png") left top no-repeat; letter-spacing: 0.35em; color: rgb(83, 83, 83);">用途</h3><div class="detail-inner-main-box clearfix child even nth-child-2 nth-child-2n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><p class="odd first-child last-child nth-child-1" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-2 nth-child-2n" style="margin: 0px; padding: 0px;">PCR</p>
<p class="odd last-child nth-child-3 nth-child-3n" style="margin: 0px; padding: 0px;"></p>
</div><h3 id="d03" class="title odd nth-child-3 nth-child-3n" style="margin: 15px 0px 0px; padding: 10px 10px 10px 20px; font-size: 1.9em; font-family: 黑体; height: 16px; background: url("../img/detail_title_bg02.png") left top no-repeat; letter-spacing: 0.35em; color: rgb(83, 83, 83);"><a name="m03" id="m03"></a>说明</h3><div class="detail-inner-main-box clearfix child even nth-child-4 nth-child-2n nth-child-4n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><p class="odd first-child last-child nth-child-1" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-2 nth-child-2n" style="margin: 0px; padding: 0px;"></p>
<table class="MsoTableGrid ke-zeroborder odd nth-child-3 nth-child-3n" border="0" cellspacing="0" style="border-collapse: collapse; border-spacing: 0px; width: 425.3pt; border-width: initial; border-style: none; border-color: initial;"><tbody><tr><td width="284" valign="top" style="margin: 0px; padding: 0px;"><p style="margin: 0px; padding: 0px;">该产品是以改良型KOD DNA polymerase（UKOD）为基础开发的PCR用2× Master Mix，兼具保真性好和扩增效率高的特点。 </p>
<p style="margin: 0px; padding: 0px;">使用改进的延伸增强剂技术，可以实现超高速PCR反应:长度在1kb以内的片段仅需1秒， 1~10kb时延伸速度为5秒/kb，10kb及以上延伸速度为10秒/kb。该酶具有很高的反应成功率，针对含肌苷(dI)或尿嘧啶(dU)的引物模板或者粗体样品都能很好地进行扩增。此外，因为预添加了抑制酶活性和3`5`外切酶活性的单抗，可以进行高特异性的热启动PCR。</p>
<div style="margin: 0px; padding: 0px;"><br/></div><br/></td><td width="283" valign="top" style="margin: 0px; padding: 0px; text-align: right;">
<img src="http://www.bio-toyobo.cn/photo/image/20180815/20180815144488168816.png" alt="" style="border: 0px;"><br/></td></tr></tbody></table><p class="even nth-child-4 nth-child-2n nth-child-4n" style="margin: 0px; padding: 0px;"></p>
<br class="odd nth-child-5 nth-child-5n"> <p class="even last-child nth-child-6 nth-child-2n nth-child-3n nth-child-6n" style="margin: 0px; padding: 0px;"></p>
</div><h3 id="d04" class="title odd nth-child-5 nth-child-5n" style="margin: 15px 0px 0px; padding: 10px 10px 10px 20px; font-size: 1.9em; font-family: 黑体; height: 16px; background: url("../img/detail_title_bg02.png") left top no-repeat; letter-spacing: 0.35em; color: rgb(83, 83, 83);"><a name="m04" id="m04"></a>特征</h3><div class="detail-inner-main-box clearfix child even nth-child-6 nth-child-2n nth-child-3n nth-child-6n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><p class="odd first-child last-child nth-child-1" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-2 nth-child-2n" style="margin: 0px; padding: 0px;">●高速PCR</p>
<p class="MsoNormal odd nth-child-3 nth-child-3n" style="margin: 0px; padding: 0px;"></p>
<table class="MsoTableGrid ke-zeroborder even nth-child-4 nth-child-2n nth-child-4n" border="0" cellspacing="0" style="border-collapse: collapse; border-spacing: 0px; width: 425.3pt; border-width: initial; border-style: none; border-color: initial;"><tbody><tr><td width="284" valign="top" style="margin: 0px; padding: 0px;">延伸时间设为1秒即可扩增1kb的片段，10kb以内延伸速度为5秒/kb，高于10kb的片段设为10秒/kb，缩短延伸时间可大幅提高PCR实验的总耗时。 <br/><div style="margin: 0px; padding: 0px;"><br/></div><br/></td><td width="283" valign="top" style="margin: 0px; padding: 0px;">
<img src="http://www.bio-toyobo.cn/photo/image/20180815/20180815145191239123.png" alt="" style="border: 0px;"><br/></td></tr></tbody></table><p class="odd nth-child-5 nth-child-5n" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-6 nth-child-2n nth-child-3n nth-child-6n" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal odd nth-child-7 nth-child-7n" style="margin: 0px; padding: 0px;">●简便</p>
<p class="MsoNormal even nth-child-8 nth-child-2n nth-child-4n nth-child-8n" style="margin: 0px; padding: 0px;">除了引物和模板之外的PCR组分都包含在预混液中，简化了配液流程，同时也提高了实验的可重复性。此外，包含电泳指示剂的KOD One<sup>TM</sup> PCR Master Mix –Blue-(Code No. KMM-201)可以在PCR反应完成后直接点样，无需另外添加Loading Dye。</p>
<p class="MsoNormal odd nth-child-9 nth-child-3n nth-child-9n" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-10 nth-child-2n nth-child-5n nth-child-10n" style="margin: 0px; padding: 0px;">●超高的保真性<br/>保真性约为Taq DNA 聚合酶的80倍，保持了KOD系列产品中保真性最高的水准</p>
<img src="http://www.bio-toyobo.cn/photo/image/20180815/20180815145288678867.png" alt="" class="odd nth-child-11 nth-child-11n" style="border: 0px;"><p class="MsoNormal even nth-child-12 nth-child-2n nth-child-3n nth-child-4n nth-child-6n nth-child-12n" style="margin: 0px; padding: 0px;">●粗样品也可高效扩增（实验例2）</p>
<p class="MsoNormal odd nth-child-13 nth-child-13n" style="margin: 0px; padding: 0px;">●可以使用含肌苷(dI)或尿嘧啶(dU)的引物和模板（实验例5）</p>
<p class="even nth-child-14 nth-child-2n nth-child-7n nth-child-14n" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal odd nth-child-15 nth-child-3n nth-child-5n nth-child-15n" style="margin: 0px; padding: 0px;"></p>
 <p class="even last-child nth-child-16 nth-child-2n nth-child-4n nth-child-8n nth-child-16n" style="margin: 0px; padding: 0px;"></p>
</div><h3 id="d06" class="title odd nth-child-7 nth-child-7n" style="margin: 15px 0px 0px; padding: 10px 10px 10px 20px; font-size: 1.9em; font-family: 黑体; height: 16px; background: url("../img/detail_title_bg02.png") left top no-repeat; letter-spacing: 0.35em; color: rgb(83, 83, 83);"><a name="m06" id="m06"></a>实验例</h3><div class="detail-inner-main-box clearfix child even last-child nth-child-8 nth-child-2n nth-child-4n nth-child-8n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><p class="MsoNormal odd first-child nth-child-1" style="margin: 0px; padding: 0px;"><strong>1. 高速PCR实验</strong></p>
<p class="even nth-child-2 nth-child-2n" style="margin: 0px; padding: 0px;">
<img src="http://www.bio-toyobo.cn/photo/image/20180815/20180815145389248924.png" alt="" style="border: 0px;"></p>
<p class="odd nth-child-3 nth-child-3n" style="margin: 0px; padding: 0px;"></p>
<p class="even nth-child-4 nth-child-2n nth-child-4n" style="margin: 0px; padding: 0px;">
<img src="http://www.bio-toyobo.cn/photo/image/20180815/20180815150054085408.png" alt="" style="border: 0px;"></p>
<p class="odd nth-child-5 nth-child-5n" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-6 nth-child-2n nth-child-3n nth-child-6n" style="margin: 0px; padding: 0px;">以人基因组DNA为模板，可进行超快速PCR扩增。结果显示，KOD One<sup>TM</sup> PCR Master Mix及KOD One<sup>TM</sup> PCR Master Mix -Blue-扩增1 kb以下的目的片段时延伸时间设为1 sec.，1 ~10 kb的目的片段时按5 sec./ kb设定可以得到清晰的扩增条带。</p>
<p class="MsoNormal odd nth-child-7 nth-child-7n" style="margin: 0px; padding: 0px;"></p>
<p class="even nth-child-8 nth-child-2n nth-child-4n nth-child-8n" style="margin: 0px; padding: 0px;"></p>
<p class="odd nth-child-9 nth-child-3n nth-child-9n" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-10 nth-child-2n nth-child-5n nth-child-10n" style="margin: 0px; padding: 0px;"><strong>2. </strong><strong>粗样品扩增</strong></p>
<img src="http://www.bio-toyobo.cn/photo/image/20180815/20180815150297279727.png" alt="" class="odd nth-child-11 nth-child-11n" style="border: 0px;"><p class="even nth-child-12 nth-child-2n nth-child-3n nth-child-4n nth-child-6n nth-child-12n" style="margin: 0px; padding: 0px;"></p>
<p class="odd nth-child-13 nth-child-13n" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-14 nth-child-2n nth-child-7n nth-child-14n" style="margin: 0px; padding: 0px;">使用KOD One<sup>TM</sup> PCR Master Mix，比较从血液、鼠尾裂解液（碱裂解液）分别扩增人β-globin基因和小鼠Thy-1基因结果。反应根据各PCR酶推荐条件进行，延伸时间5 sec./ kb。</p>
<p class="MsoNormal odd nth-child-15 nth-child-3n nth-child-5n nth-child-15n" style="margin: 0px; padding: 0px;">结果显示，只有KOD One<sup>TM</sup> PCR Master Mix能够得到明确的条带。</p>
<p class="MsoNormal even nth-child-16 nth-child-2n nth-child-4n nth-child-8n nth-child-16n" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal odd nth-child-17 nth-child-17n" style="margin: 0px; padding: 0px;">3.<strong> </strong><strong>逆转录反应液（cDNA）的添加量对扩增的影响</strong></p>
<p class="MsoNormal even nth-child-18 nth-child-2n nth-child-3n nth-child-6n nth-child-9n nth-child-18n" style="margin: 0px; padding: 0px;">逆转录反应液中残留的RNA对PCR有抑制作用，过量添加cDNA模板会使扩增效果变差。KOD One<sup>TM</sup> PCR Master Mix不易受RNA的抑制作用。为了确认可引入的cDNA的量，通过加入不同量的cDNA模板，检测扩增TFR基因的效果。与原来的产品及其他公司的产品相比，即使添加较多的cDNA也能得到良好的扩增。</p>
<p class="odd nth-child-19 nth-child-19n" style="margin: 0px; padding: 0px;"></p>
<img src="http://www.bio-toyobo.cn/photo/image/20180815/2018081515030842842.png" alt="" class="even nth-child-20 nth-child-2n nth-child-4n nth-child-5n nth-child-10n nth-child-20n" style="border: 0px;"></div><div class="detail-inner-main-box clearfix child even last-child nth-child-8 nth-child-2n nth-child-4n nth-child-8n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><br/></div><div class="detail-inner-main-box clearfix child even last-child nth-child-8 nth-child-2n nth-child-4n nth-child-8n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><br/></div>

TOYOBO .KMM-201 KOD ONE TM PCR Mix-Blue 1 ml ×5支

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