TOYOBO.KFX-201 KOD FX Neo 200U×1支

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货品编号：TOYOBO.KFX-201

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品牌：Toyobo
所属分类：辅助货品

<p>     货品编号：  TOYOBO.KFX-201<span style="white-space: pre;">	</span></p>
<p>     品牌：  TOYOBO</p>
<p>     品名：  KOD FX Neo<span style="white-space: pre;">	</span></p>
<p>     规格：  200U×1支</p>
<p> </p>
<div><h3 id="d02" class="title odd first-child nth-child-1" style="margin: 0px; padding: 10px 10px 10px 20px; font-size: 1.9em; font-family: 黑体; height: 16px; background: url("../img/detail_title_bg02.png") left top no-repeat; letter-spacing: 0.35em; color: rgb(83, 83, 83);">用途</h3><div class="detail-inner-main-box clearfix child even nth-child-2 nth-child-2n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><p class="odd first-child last-child nth-child-1" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-2 nth-child-2n" style="margin: 0px; padding: 0px;"><strong>PCR</strong><strong></strong></p>
<p class="MsoNormal odd nth-child-3 nth-child-3n" style="margin: 0px; padding: 0px;"><strong><br/></strong></p>
<p class="MsoNormal even nth-child-4 nth-child-2n nth-child-4n" style="margin: 0px; padding: 0px;"><strong><br/></strong></p>
<p class="odd last-child nth-child-5 nth-child-5n" style="margin: 0px; padding: 0px;"></p>
</div><h3 id="d03" class="title odd nth-child-3 nth-child-3n" style="margin: 15px 0px 0px; padding: 10px 10px 10px 20px; font-size: 1.9em; font-family: 黑体; height: 16px; background: url("../img/detail_title_bg02.png") left top no-repeat; letter-spacing: 0.35em; color: rgb(83, 83, 83);"><a name="m03" id="m03"></a>说明</h3><div class="detail-inner-main-box clearfix child even nth-child-4 nth-child-2n nth-child-4n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><p class="odd first-child last-child nth-child-1" style="margin: 0px; padding: 0px;"></p>
<p class="even nth-child-2 nth-child-2n" style="margin: 0px; padding: 0px;"></p>
<table class="odd nth-child-3 nth-child-3n" style="border-collapse: collapse; border-spacing: 0px;"><tbody><tr><td style="margin: 0px; padding: 0px;">KOD FX Neo是在高成功率PCR酶KOD FX的基础上添加了「延伸增强剂」而开发的PCR酶。在保持高扩增效率的同时，进一步提高了对难扩增序列、微量模板DNA、长目的片段及粗样品的扩增效率。<br/><p style="margin: 0px; padding: 0px;"></p>
</td><td style="margin: 0px; padding: 0px;"><blockquote style="margin: 0px; padding: 0px;"></blockquote></td><td style="margin: 0px; padding: 0px;"><blockquote style="margin: 0px; padding: 0px;"></blockquote></td><td style="margin: 0px; padding: 0px;"><blockquote style="margin: 0px; padding: 0px;"></blockquote></td><td style="margin: 0px; padding: 0px;">
<img src="http://www.bio-toyobo.cn/photo/image/20171026/20171026145142764276.jpg" alt="" style="border: 0px;"></td></tr></tbody></table><p class="even nth-child-4 nth-child-2n nth-child-4n" style="margin: 0px; padding: 0px;"></p>
<br class="odd nth-child-5 nth-child-5n"> <p class="even last-child nth-child-6 nth-child-2n nth-child-3n nth-child-6n" style="margin: 0px; padding: 0px;"></p>
</div><h3 id="d04" class="title odd nth-child-5 nth-child-5n" style="margin: 15px 0px 0px; padding: 10px 10px 10px 20px; font-size: 1.9em; font-family: 黑体; height: 16px; background: url("../img/detail_title_bg02.png") left top no-repeat; letter-spacing: 0.35em; color: rgb(83, 83, 83);"><a name="m04" id="m04"></a>特征</h3><div class="detail-inner-main-box clearfix child even nth-child-6 nth-child-2n nth-child-3n nth-child-6n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><p class="odd first-child last-child nth-child-1" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-2 nth-child-2n" style="margin: 0px; padding: 0px;"> <strong>●</strong><strong>特征1.　延伸性UP</strong></p>
<p class="MsoNormal odd nth-child-3 nth-child-3n" style="margin: 0px; padding: 0px;">・以基因组DNA为模板最大可扩增40kb的片段<br/>・实现了30 sec/kb的高速循环（粗样品推荐1min/kb）<br/>・最适用于高GC含量目的片段的扩增<br/>此外，还适用于使用抗体的<a href="http://www.bio-toyobo.cn/product_detail_25.html" style="text-decoration-line: none; color: rgb(41, 41, 41);">热启动法</a>，可进行特异性良好的扩增。</p>
<p class="MsoNormal even nth-child-4 nth-child-2n nth-child-4n" style="margin: 0px; padding: 0px;"><br/><strong>●</strong><strong>特征2.　对粗样品的扩增性能UP</strong><br/>・提高了对植物裂解液、小鼠尾巴的扩增效率<br/>・降低了土壤成分、食品成分等的PCR抑制效果<br/>・与基础版产品KOD FX一样，可对革兰氏阳性菌、酵母、霉菌等直接进行PCR</p>
<p class="MsoNormal odd nth-child-5 nth-child-5n" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-6 nth-child-2n nth-child-3n nth-child-6n" style="margin: 0px; padding: 0px;"></p>
 <p class="odd last-child nth-child-7 nth-child-7n" style="margin: 0px; padding: 0px;"></p>
</div><h3 id="d05" class="title odd nth-child-7 nth-child-7n" style="margin: 15px 0px 0px; padding: 10px 10px 10px 20px; font-size: 1.9em; font-family: 黑体; height: 16px; background: url("../img/detail_title_bg02.png") left top no-repeat; letter-spacing: 0.35em; color: rgb(83, 83, 83);"><a name="m05" id="m05"></a>原理</h3><div class="detail-inner-main-box clearfix child even nth-child-8 nth-child-2n nth-child-4n nth-child-8n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><p class="odd first-child last-child nth-child-1" style="margin: 0px; padding: 0px;"></p>
<p class="MsoNormal even nth-child-2 nth-child-2n" style="margin: 0px; padding: 0px;">KOD DNA Polymerase具有卓越的延伸性，同时对粗样品成分中的抑制物质有很强的抵抗力。<br/>KOD FX就是利用这种特性而开发出来的高成功率PCR酶，该酶在难扩增序列及粗样品的扩增方面受到了广泛的好评。<br/>然而，以KOD FX为代表的以往的PCR酶在20~30个循环以后，很容易出现扩增无法持续的<停滞现象>，PCR的功能无法完全发挥出来。</p>
<p class="odd nth-child-3 nth-child-3n" style="margin: 0px; padding: 0px;">【KOD FX Neo】是在KOD FX的基础上，应用了本公司新开发的【延伸增强剂】技术，从而成功地抑制了<停滞现象>，进一步提高了对长目的片段、难扩增序列及粗样品等的扩增效率。</p>
<p class="even nth-child-4 nth-child-2n nth-child-4n" style="margin: 0px; padding: 0px;">
<img src="http://www.bio-toyobo.cn/photo/image/20171026/20171026153636943694.jpg" alt="" style="border: 0px;"></p>
<p class="odd nth-child-5 nth-child-5n" style="margin: 0px; padding: 0px;"></p>
<p class="even last-child nth-child-6 nth-child-2n nth-child-3n nth-child-6n" style="margin: 0px; padding: 0px;"></p>
</div><h3 id="d06" class="title odd nth-child-7 nth-child-7n nth-child-9 nth-child-3n nth-child-9n" style="margin: 15px 0px 0px; padding: 10px 10px 10px 20px; font-size: 1.9em; font-family: 黑体; height: 16px; background: url("../img/detail_title_bg02.png") left top no-repeat; letter-spacing: 0.35em; color: rgb(83, 83, 83);"><a name="m06" id="m06"></a>实验例</h3><div class="detail-inner-main-box clearfix child even last-child nth-child-8 nth-child-2n nth-child-4n nth-child-8n nth-child-10 nth-child-5n nth-child-10n" style="margin: 0px; padding: 10px; zoom: 1; font-size: 1.4em; line-height: 1.6; color: rgb(83, 83, 83); font-family: 黑体;"><h4 class="odd first-child nth-child-1" style="margin: 0px; padding: 0px; font-size: 14px; font-weight: bolder;">1. 扩增长度的比较</h4><p class="even nth-child-2 nth-child-2n" style="margin: 0px; padding: 0px;">使用KOD FX Neo及以往的产品，以人基因组DNA为模板进行长链目的片段的扩增。结果显示，只有使用KOD FX Neo的情况下，才可以确认扩增40kb长的目的片段。另外，与以往产品相比，KOD FX Neo只需一半的延伸时间(30sec/kb) *。因此，可以极大地缩短反应时间。</p>
<p class="odd nth-child-3 nth-child-3n" style="margin: 0px; padding: 0px;">*粗样品扩增时建议按1min/kb进行。</p>
<h4 class="even nth-child-4 nth-child-2n nth-child-4n" style="margin: 0px; padding: 0px; font-size: 14px; font-weight: bolder;">
<img src="http://www.bio-toyobo.cn/photo/image/20171026/20171026145765856585.jpg" alt="" style="border: 0px;"></h4><h4 class="odd nth-child-5 nth-child-5n" style="margin: 0px; padding: 0px; font-size: 14px; font-weight: bolder;">2. 检测灵敏度的比较</h4><p class="even nth-child-6 nth-child-2n nth-child-3n nth-child-6n" style="margin: 0px; padding: 0px;">以人基因组DNA为模板进行检测灵敏度的比较。结果可见，与原产品相比，KOD FX Neo的灵敏度提高了约10倍。</p>
<p class="odd nth-child-7 nth-child-7n" style="margin: 0px; padding: 0px;">
<img src="http://www.bio-toyobo.cn/photo/image/20171026/20171026145895679567.jpg" alt="" style="border: 0px;"></p>
<h4 class="even nth-child-8 nth-child-2n nth-child-4n nth-child-8n" style="margin: 0px; padding: 0px; font-size: 14px; font-weight: bolder;">3. 扩增的比较</h4><p class="odd nth-child-9 nth-child-3n nth-child-9n" style="margin: 0px; padding: 0px;">以碱裂解法制备的鼠尾裂解液为样品，扩增3种小鼠的基因。结果可见，只有用KOD FX Neo才能得到良好的扩增。像这样，KOD FX Neo能够对样品进行直接PCR，可以省略掉繁杂的纯化过程。</p>
<p class="even nth-child-10 nth-child-2n nth-child-5n nth-child-10n" style="margin: 0px; padding: 0px;">
<img src="http://www.bio-toyobo.cn/photo/image/20171026/20171026145950465046.jpg" alt="" style="border: 0px;"></p>
<p class="odd nth-child-11 nth-child-11n" style="margin: 0px; padding: 0px;">使用96孔PCR板的鼠尾处理方法(碱裂解法)</p>
<blockquote class="even nth-child-12 nth-child-2n nth-child-3n nth-child-4n nth-child-6n nth-child-12n" style="margin: 0px; padding: 0px;"><p style="margin: 0px; padding: 0px;">将鼠尾(约3 mm)放入离心管中。<br/>↓←添加50 mM NaOH 180μl，盖上盖子，用Vortex充分振荡<br/>↓spin down（轻轻地振荡使液体落到管下部)<br/>↓95℃, 10 min.孵育(使用Thermo cycler)<br/>↓←添加1 M Tris-HCl (pH 8.0) 20μl，盖上盖子，用Vortex充分振荡<br/>↓spin down（轻轻地振荡使液体落到管下部)<br/>取0.5〜2μl上清(模板)添加到50μl反应体系中进行PCR反应</p>
<p style="margin: 0px; padding: 0px;">※鼠尾切片，请切得小一些，使之能够浸没在裂解液中。<br/>※请注意热碱。<br/>※处理后，鼠尾不会完全溶解。大约只能溶解鼠尾的表面。<br/>※本方法，可使用1.5m的离心管。</p>
</blockquote><br class="odd nth-child-13 nth-child-13n"><h4 class="even nth-child-14 nth-child-2n nth-child-7n nth-child-14n" style="margin: 0px; padding: 0px; font-size: 14px; font-weight: bolder;">4.腐殖酸添加实验</h4><p class="odd nth-child-15 nth-child-3n nth-child-5n nth-child-15n" style="margin: 0px; padding: 0px;">腐殖酸(humic acid)是存在于腐殖土及土壤中的红褐色或黑褐色有机物质，对PCR有抑制作用。<br/>通常进行的DNA纯化过程中不能去除这种腐殖酸，因此，以环境、生态体系中的样品作为模板进行PCR非常困难。<br/>这里，以人基因组DNA中混入腐殖酸为例，探讨了其对PCR的抑制作用。结果显示，KOD FX Neo对抗腐殖酸的能力最强。<br/>因此，使用KOD FX Neo可以对环境、生态体系中的样品进行PCR。</p>
<p class="even nth-child-16 nth-child-2n nth-child-4n nth-child-8n nth-child-16n" style="margin: 0px; padding: 0px;">
<img src="http://www.bio-toyobo.cn/photo/image/20171026/20171026150012311231.jpg" alt="" style="border: 0px;"></p>
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<p class="even nth-child-16 nth-child-2n nth-child-4n nth-child-8n nth-child-16n" style="margin: 0px; padding: 0px;"></p>
</div></div>

TOYOBO.KFX-201 KOD FX Neo 200U×1支

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