vazyme.R223-00 HiScript ® II Q RT SuperMix for qPCR（+g DNA wiper） 5 rxn

vazyme.R223-00 HiScript ® II Q RT SuperMix for qPCR（+g DNA wiper） 5 rx

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货品编号：vazyme.R223-00

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所属分类：辅助货品

<div>     货品编号：  vazyme.R223-00	     品牌：  vazyme     品名：  HiScript ® II Q RT SuperMix for qPCR（+g DNA wiper）	     规格：  5 rxn</div> <div><div id="c_portalResProduct_detail-15958157317764033" class="c_portalResProduct_detail-04001" loaded="true" style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0px auto; min-width: 250px; max-width: 100%; max-height: 100%; width: auto; height: auto; overflow: visible; min-height: 0px; color: rgb(89, 89, 89); font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 14px; word-break: break-word !important;"><div class="e_box d_ProductDetail p_ProductDetail" data-ename="产品详情框架" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; width: 927.013px; height: auto; flex-flow: row wrap; display: flex; align-items: flex-start;"><div class="e_box d_DescriptionBoxA p_DescriptionBoxA " data-ename="详情信息区" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 20px 0px 0px; width: 927.013px; height: auto; overflow: visible; clear: both;"><div class="e_box d_DescriptionBigbox p_DescriptionBigbox borderT_default" data-ename="页签内容区" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; border-top: 1px solid rgb(217, 217, 217); width: auto; height: auto; clear: both; overflow: hidden;"><div id="contentDiv" class="e_box d_DescribeContent p_DescribeContent" data-ename="描述内容区" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px;"><div class="e_HtmlEditor d_description" style="box-sizing: border-box; animation-fill-mode: both; padding: 20px 0px; margin: 0px; width: auto; height: auto; word-break: break-word !important;"><div class="reset_style js-reset_style js-adapMobile " style="box-sizing: border-box; animation-fill-mode: both; word-break: break-all; padding: 0px; margin: 0px; overflow-wrap: break-word; line-height: normal;">HiScript<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 15px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">® II Q RT SuperMix for qPCR (+gDNA wiper)
预混液形式的两步法RT-qPCR 首选试剂（去基因组）

<img alt="" class="imageResponse js-onerror" id="530" src="https://www.vazyme.com/repository/image/b8086d06-0202-481c-9739-4f43a705a612.png?k=1593524907000" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; border: 0px; vertical-align: middle; width: 890px; height: 80px;">
<p align="left" style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 2em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">基于高效的HiScript<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">® II Reverse Transcriptase，逆转录效率更高
<p align="left" style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 2em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">Anchored Oligo (dT)<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; bottom: -0.25em;"> 23  VN 设计独特，确保反应特异性，提高第一链cDNA 合成效率和成功率
<p align="left" style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 2em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">即用型的SuperMix 预混液使用方便省时
<p align="left" style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 2em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">gDNA wiper Mix 可迅速彻底去除基因组污染

<img alt="" class="imageResponse js-onerror" id="526" src="https://www.vazyme.com/repository/image/19d70623-3cf4-4e83-9b21-e6b13c7e1c36.png?k=1593524907000" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; border: 0px; vertical-align: middle; width: 890px; height: 80px;">

<img src="https://2005205005-site.pool5.yun300.cn/repository/image/8dXNpnfzQGav3C6i1rYq7A.png" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; border: 0px; vertical-align: middle; width: 648.9px; height: 668.162px;">
 
<p style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 2em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">以HeLa 细胞RNA 为模板，使用Vazyme #R223 和Supplier Ta、Supplier Tg 同类产品，在各自说明书所提供的反应体系及反应条件下进行逆转录，然后用qPCR 检测基因ACTB 和CBR3。结果表明与同类产品相比，Vazyme #R223 在扩增效率和扩增线性上表现都较优异。
<p style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 0em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">
<img alt="" class="imageResponse js-onerror" id="524" src="https://www.vazyme.com/repository/image/025ecb51-5b91-49fe-a85e-d79bfb3bdebc.png?k=1593524907000" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; border: 0px; vertical-align: middle; width: 890px; height: 80px;">
<p style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 2em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">本产品是以高性价比的HiScript<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">® II Reverse Transcriptase 为基础的两步法qPCR 试剂盒。试剂盒中的4 × gDNA wiper Mix 可彻底去除残留的基因组DNA 污染，保证后续定量结果更加可靠。5 × HiScript<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">®<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-family: 微软雅黑体, tahoma, arial, sans-serif; letter-spacing: 0em; text-indent: 2em;">II qRT SuperMix II 中含有逆转录反应所需的所有组分，加入模板RNA 和水即可迅速进行反应，同时终止gDNA wiper 作用，保证cDNA 的完整性。本产品针对qPCR 进行特别优化，逆转录产物兼容SYBR Green 和探针法qPCR，可以根据实验目的，选择相应的试剂配合使用，进行高性能的基因表达分析。
<p style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 0em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">
<img alt="" class="imageResponse js-onerror" id="529" src="https://www.vazyme.com/repository/image/96fb9b9b-8e4d-4ca4-9999-d6a8215b848a.png?k=1593524907000" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; border: 0px; vertical-align: middle; width: 890px; height: 80px;">
<table border="1" cellpadding="0" cellspacing="0" width="100%" style="animation-fill-mode: both; padding: 0px; margin: 0px; border-collapse: collapse; border-spacing: 0px; background-color: white; width: 927.013px; border-width: initial; border-style: none; border-color: initial; word-break: break-word !important;"><tbody style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px;"><tr style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px;"><td style="box-sizing: border-box; animation-fill-mode: both; padding: 0cm 5.4pt; margin: 0px; width: 309px; border-width: 1pt; border-style: solid; border-color: windowtext; vertical-align: top; word-break: break-word !important;"><p align="left" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0em 0px; list-style-type: none; font-size: 14px; text-indent: 0em; letter-spacing: 0em; line-height: 2em;">组分
</td><td style="box-sizing: border-box; animation-fill-mode: both; padding: 0cm 5.4pt; margin: 0px; width: 259px; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; vertical-align: top; word-break: break-word !important;"><p align="left" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0em 0px; list-style-type: none; font-size: 14px; text-indent: 0em; letter-spacing: 0em; line-height: 2em;">R223-01 100 rxn (20 μl/rxn)
</td></tr><tr style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px;"><td style="box-sizing: border-box; animation-fill-mode: both; padding: 0cm 5.4pt; margin: 0px; width: 309px; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; vertical-align: top; word-break: break-word !important;"><p align="left" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0em 0px; list-style-type: none; font-size: 14px; text-indent: 0em; letter-spacing: 0em; line-height: 2em;">RNase free ddH<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; bottom: -0.25em;">2O
</td><td style="box-sizing: border-box; animation-fill-mode: both; padding: 0cm 5.4pt; margin: 0px; width: 259px; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; vertical-align: top; word-break: break-word !important;"><p align="left" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0em 0px; list-style-type: none; font-size: 14px; text-indent: 0em; letter-spacing: 0em; line-height: 2em;">2×1 ml  
</td></tr><tr style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px;"><td style="box-sizing: border-box; animation-fill-mode: both; padding: 0cm 5.4pt; margin: 0px; width: 309px; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; vertical-align: top; word-break: break-word !important;"><p align="left" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0em 0px; list-style-type: none; font-size: 14px; text-indent: 0em; letter-spacing: 0em; line-height: 2em;">4 × gDNA wiper Mix
</td><td style="box-sizing: border-box; animation-fill-mode: both; padding: 0cm 5.4pt; margin: 0px; width: 259px; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; vertical-align: top; word-break: break-word !important;"><p align="left" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0em 0px; list-style-type: none; font-size: 14px; text-indent: 0em; letter-spacing: 0em; line-height: 2em;">400 μl
</td></tr><tr style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px;"><td style="box-sizing: border-box; animation-fill-mode: both; padding: 0cm 5.4pt; margin: 0px; width: 309px; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; vertical-align: top; word-break: break-word !important;"><p align="left" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0em 0px; list-style-type: none; font-size: 14px; text-indent: 0em; letter-spacing: 0em; line-height: 2em;">5 × HiScript<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">® II qRT SuperMix II <span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">a
</td><td style="box-sizing: border-box; animation-fill-mode: both; padding: 0cm 5.4pt; margin: 0px; width: 259px; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; vertical-align: top; word-break: break-word !important;"><p align="left" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0em 0px; list-style-type: none; font-size: 14px; text-indent: 0em; letter-spacing: 0em; line-height: 2em;">400 μl
</td></tr><tr style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px;"><td style="box-sizing: border-box; animation-fill-mode: both; padding: 0cm 5.4pt; margin: 0px; width: 309px; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; vertical-align: top; word-break: break-word !important;"><p align="left" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0em 0px; list-style-type: none; font-size: 14px; text-indent: 0em; letter-spacing: 0em; line-height: 2em;">5 × No RT Control Mix <span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">b
</td><td style="box-sizing: border-box; animation-fill-mode: both; padding: 0cm 5.4pt; margin: 0px; width: 259px; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; vertical-align: top; word-break: break-word !important;"><p align="left" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0em 0px; list-style-type: none; font-size: 14px; text-indent: 0em; letter-spacing: 0em; line-height: 2em;">40 μl
</td></tr></tbody></table><p align="left" style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 0em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">a.   包含Buffer、dNTP、HiScript<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">® II Reverse Transcriptase、RNase inhibitor、Random primers/Oligo (dT)<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; bottom: -0.25em;">23VN primer mix。
<p align="left" style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 0em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">注意：与HiScript<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">® II Q RT SuperMix  for qPCR (R222-01)中所带的5 ×HiScript<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">® II qRT SuperMix成分不同，不可以混用。
<p align="left" style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 0em 0px; list-style-type: none; text-indent: 0em; letter-spacing: 0em; line-height: 2em; word-break: break-word !important;">b.  除不含HiScript<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">® II Reverse Transcriptase外，其余成分与5 ×HiScript<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">® II qRT SuperMix II相同，用于配制No RT对照反应,排除基因组残留。

<img alt="" class="imageResponse js-onerror" id="525" src="https://www.vazyme.com/repository/image/bcb389c5-1539-4af8-8272-3dd3743fc14b.png?k=1593524907000" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; border: 0px; vertical-align: middle; width: 890px; height: 80px;">
<span style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; color: rgb(105, 105, 105); font-family: 微软雅黑体, tahoma, arial, sans-serif; font-size: 16px;">-20℃保存
</div></div></div></div></div></div><div class="e_box p_shade" data-ename="遮罩容器" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; width: 927.013px; height: 0px; background-color: rgba(0, 0, 0, 0.6);"></div></div><div id="w_fimg-1595385712152" class="w_fimg-000" style="box-sizing: border-box; animation-fill-mode: both; padding: 0px; margin: 100px 0px 0px; position: relative; width: 927.013px; height: auto; min-height: 0px; max-width: none; min-width: 0px; max-height: none; color: rgb(89, 89, 89); font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 14px; word-break: break-word !important;"><div class="w_img animated " style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; animation-duration: 1s; width: 927.013px; height: 84.9px; position: relative; min-height: inherit;"><div class="e_box e_box-000 p_box" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; width: 927.013px; height: 84.9px; text-align: center; position: static;"></div><div class="e_box e_box-000 p_box" style="box-sizing: border-box; animation-fill-mode: both; word-break: break-word !important; padding: 0px; margin: 0px; width: 927.013px; height: 84.9px; text-align: center; position: static;"> </div></div></div></div>

vazyme.R223-00 HiScript ® II Q RT SuperMix for qPCR（+g DNA wiper） 5 rxn

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