Fermentas EL0011 T4 DNA Ligase 1000units

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

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货品编号：Fermentas.EL0011

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品牌：Fermentas
所属分类：分子生物学试剂

货品编号：  Fermentas.EL0011
     品牌：  Fermentas
     品名：  T4 DNA Ligase
     规格：  1000units/支
 
     产品描述      Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between      juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.      The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.      It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
      T4 DNA Ligase requires ATP as a cofactor.
 
     Highlights      • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)      • Fast—sticky-end ligation is completed in 10 minutes at room temperature      • Supplied with PEG solution for efficient blunt-end ligation      Applications      • Cloning of restriction enzyme generated DNA fragments      • Cloning of PCR products      • Joining of double-stranded oligonucleotide linkers or adaptors to DNA      • Site-directed mutagenesis      • Amplified fragment length polymorphism (AFLP)      • Ligase-mediated RNA detection (see Reference 3)      • Nick repair in duplex DNA, RNA or DNA/RNA hybrids      • Self-circularization of linear DNA.      Includes      • T4 DNA Ligase      • 10X T4 DNA Ligase Buffer      • 50% PEG Solution      Notes      • Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, 
       incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes 
       and chill on ice prior to electrophoresis.      • The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.      • Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction. 
       The extracted DNA can be further  precipitated with ethanol.

Fermentas EL0011 T4 DNA Ligase 1000units

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