NEB T1020S Monarch™ DNA Gel Extraction Kit 50 preps

The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels.

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货品编号：NEB.T1020S

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品牌：NEB
所属分类：分子生物学试剂

货品编号：  NEB.T1020S
      品牌： NEB
      品名： Monarch™ DNA Gel Extraction Kit
 
      产品描述
      The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from 
      agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure 
      zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers
      provided have been optimized, anddo not require monitoring of pH. Unlike other kits, there is no need to add isopropanol 
      to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, 
      DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use 
      significantly less plastic and responsibly-sourced, recyclable packaging 
 
     Monarch DNA Cleanup Column Design      
<img width="450" height="275" alt="" src="http://www.neb-china.com/userfiles/Monarch_CleanupColumnDesign.png">      Monarch columns are designed for performance      
<img width="385" height="175" alt="" src="http://www.neb-china.com/userfiles/Monarch_BufferRetention.png">
     Monarch columns are designed without a frit, which eliminates buffer
     retention and the risk of carryover contamination, providing fast, worry-free DNA purification.      Advantages: 
     Elute in as little as 6 μl
     Prevent buffer retention and salt carry-over with optimized column design
     Save time with fast, user-friendly protocols
      No need to monitor pH or add isopropanol
     Buffers and columns available separately
     Significantly less plastic used when compared with other kits
     Responsibly-sourced and recyclable packaging
     Specifications
     
<img src="http://solelybio.com/public/images/fa/51/81/2f90bc69c608fab856fa9cec3e5f38f051f94fce.jpg?1464689578#w">
     Monarch DNA Gel Extraction Kit Protocol       
<img width="420" height="786" alt="" src="http://www.neb-china.com/userfiles/T1020_GelExtractionKitWorkflow.png">      DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and 
     purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications      
<img width="600" height="261" alt="" src="http://www.neb-china.com/userfiles/MonarchGelExtractionData1.png">      One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with
     different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and 
     purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume.       Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights.       
<img width="375" height="283" alt="" src="http://www.neb-china.com/userfiles/MonarchGelExtractionData2.png">
     A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the 
     mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and 
     processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved 
     on a new gel with the remainder of the original mixture for comparison.       DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated.      
<img width="340" height="309" alt="" src="http://www.neb-china.com/userfiles/MonarchGelExtractionData3.png">      Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, 
     and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) 
     was ligated using the Blunt/TA Ligase Master Mix . Representative samples from 5 replicates were 
     resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder .
<h4 style="margin: 1em 0px 0px; padding: 0px; border: 0px; font-size: 13px; color: rgb(76, 76, 76); font-family: Arial, Helvetica, sans-serif; font-stretch: normal; clear: both;">     Kit Components</h4>     The following reagents are supplied with this product:
     
<img src="http://solelybio.com/public/images/d9/29/8f/a25d91fe30399edc32cba7c84900ac8c12b57e87.jpg?1464690044#w">
<h1 style="clear: both; margin-top: 10px; font-weight: bold; font-size: 12px; margin-bottom: 10px; margin-left: 15px; width: 560px; color: #ca9932; border-bottom: #ccc 1px solid; font-family: Arial; height: 18px">Advantages and Features</h1><div style="padding-left: 15px; width: 560px; word-break: break-all; line-height: 24px; word-wrap: break-word"><h4 style="margin: 1em 0px 0px; padding: 0px; border: 0px; font-size: 13px; color: rgb(76, 76, 76); font-family: Arial, Helvetica, sans-serif; font-stretch: normal; clear: both;">Features</h4><ul style="margin: 0px 0px 1em 1em; padding: 0px; border: 0px; list-style-image: url(https://www.neb.com/sites/nebus/_resources/img/css/bullet.png); color: rgb(76, 76, 76); font-family: Arial, Helvetica, sans-serif; font-size: 13px; line-height: 17.3333px;"><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Elute in as little as 6 μl</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Prevent buffer retention and salt carry-over with optimized column design</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Save time with fast, user-friendly protocols</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">No need to monitor pH or add isopropanol</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Buffers and columns available separately</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Significantly less plastic used when compared with other kits</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Responsibly-sourced and recyclable packaging</li>
</ul>
 
</div><h1 style="clear: both; margin-top: 10px; font-weight: bold; font-size: 12px; margin-bottom: 10px; margin-left: 15px; width: 560px; color: #ca9932; border-bottom: #ccc 1px solid; font-family: Arial; height: 18px">Properties and Usage</h1><div style="padding-left: 15px; width: 560px; word-break: break-all; line-height: 24px; word-wrap: break-word"><h4 style="margin: 1em 0px 0px; padding: 0px; border: 0px; font-size: 13px; color: rgb(76, 76, 76); font-family: Arial, Helvetica, sans-serif; font-stretch: normal; clear: both;">Storage Temperature</h4>25°C 
</div><h1 style="clear: both; margin-top: 10px; font-weight: bold; font-size: 12px; margin-bottom: 10px; margin-left: 15px; width: 560px; color: #ca9932; border-bottom: #ccc 1px solid; font-family: Arial; height: 18px">Troubleshooting</h1><div style="padding-left: 15px; width: 560px; word-break: break-all; line-height: 24px; word-wrap: break-word"><h4 style="margin: 1em 0px 0px; padding: 0px; border: 0px; font-size: 13px; color: rgb(76, 76, 76); font-family: Arial, Helvetica, sans-serif; font-stretch: normal; clear: both;">Troubleshooting</h4> Low DNA Yield 
<ul style="margin: 0px 0px 1em 1em; padding: 0px; border: 0px; list-style-image: url(https://www.neb.com/sites/nebus/_resources/img/css/bullet.png); color: rgb(76, 76, 76); font-family: Arial, Helvetica, sans-serif; font-size: 13px; line-height: 17.3333px;"><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Reagents added incorrectly. Check protocol to ensure correct buffer reconstitution, order of addition for buffers and proper handling of column flow-through and eluents.</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Gel slice not fully dissolved. Small clumps of agarose may clog the column or interfere with DNA binding. Be sure to incubate the gel slice in the Monarch Gel Dissolving Buffer for the specified time and within the proper temperature range. Mix the sample and inspect periodically to monitor dissolution of the agarose.</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Gel dissolved above 60°C. The DNA may become denatured if incubated at higher temperatures than the specified range of 37–55°C.</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Incomplete elution during prep. Ensure the DNA Elution Buffer is delivered directly to the center of the column so that the matrix is completely covered and elution is efficient. Larger elution volumes and longer incubation times can increase yield of DNA off the column at the cost of dilution of the sample and increased processing times. For typical fragments below 10 kb, the recommended elution volumes and incubation times should be sufficient, unless the maximal yield is desired. For the purification of larger fragments, heating the DNA Elution Buffer to 50°C prior to eluting and extending the incubation time after buffer addition to 5 minutes can improve yield. Additionally, multiple rounds of elution can be employed to increase the amount of DNA eluted, at the expense of dilution of the sample.</li>
</ul>
Low DNA Performance 
<ul style="margin: 0px 0px 1em 1em; padding: 0px; border: 0px; list-style-image: url(https://www.neb.com/sites/nebus/_resources/img/css/bullet.png); color: rgb(76, 76, 76); font-family: Arial, Helvetica, sans-serif; font-size: 13px; line-height: 17.3333px;"><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Gel slice not fully dissolved. Undissolved agarose may leach salts into the eluted DNA. Be sure to incubate the gel slice and the Monarch Gel Dissolving Buffer mixture for the specified time and temperature. Mix the sample and inspect periodically to monitor dissolving of the agarose.</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Ethanol has been carried over. Ensure final wash spin time is 1 minute to ensure complete removal of the wash buffer from the column and be careful when transferring the column to a new tube for elution step to ensure column tip does not contact column flow-through.</li><li style="margin: 0px 0px 0.5em; padding: 0px; border: 0px;">Trace amounts of salts that produce low OD260/230 ratios can also be carried over during the elution step. Be careful when transferring column to new tube for elution step to ensure the column tip does not contact column flow-through.</li>
</ul>
</div><strike> </strike>

NEB T1020S Monarch™ DNA Gel Extraction Kit 50 preps

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