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BioAssay Systems EnzyChrom™磷脂检测试剂盒,100T,储存温度:4,-20°C;
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品牌:BioAssay Systems
运输:冷藏
储存:4,-20°C 【点此查看PDF技术文档】
Application
• For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.
Key Features
• Sensitive. Use 20 μL samples. Linear detection range: colorimetric assay 3 - 200 μM, fluorimetric assay 0.6 - 20 μM phospholipid.
Method
• OD570nm, or FL530/585nm
Samples
• Biological
Species
• All
Procedure
• 20 min
Size
• 100 tests
Detection Limit
• OD, FL: 3, 0.6 μM
Shelf Life
• 6 months
More Details
• Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. BioAssay Systems’ method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.
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