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Thermo Scientific FastDigest DpnI restriction enzyme recognizes Gm6A^TC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: MalI.
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货品编号: Fermentas.FD1704
品牌: Fermentas
品名: FastDigest DpnI
规格: 100rxns
产品描述
Thermo Scientific FastDigest DpnI restriction enzyme recognizes Gm6A^TC site and cuts best at 37°C in 5–15
minutes using universal FastDigest Buffer. Isoschizomers: MalI.
Thermo Scientific FastDigest DpnI is one of an advanced line of fast restriction enzymes that are all 100% active
in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any
need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for
a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes.
DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase
all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added
to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading
of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
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