Fermentas FD0303 FASTDIGEST ECO32I EA 200 reactions

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货品编号：Fermentas.FD0303

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品牌：Fermentas
所属分类：分子生物学试剂

货品编号：  Fermentas.FD0303
     品牌：  Fermentas
     品名：  FASTDIGEST ECO32I EA   
     规格：  200 reactions
 
     产品描述
     Thermo Scientific FastDigest Eco32I restriction enzyme recognizes GAT^ATC site and cuts best at 37°C in 5–15 minutes 
     using universal FastDigest Buffer. Isoschizomers: EcoRV, EcoRV.
 
     Thermo Scientific FastDigest Eco32I is one of an advanced line of fast restriction enzymes that are all 100% active in the
     universal FastDigest and FastDigest Green reaction buffers.
 
     The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for 
     buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme 
     activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, 
     such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest 
     Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
 
     For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of 
     digestion reaction products on gels.
 
     Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects. 
 
     Features      • 100% activity of all FastDigest enzymes in the universal buffer      • 100% buffer compatibility with downstream applications      • Complete digestion in 5–15 minutes      • Direct loading on gels      • No star activity      • 176 FastDigest enzymes available 
 
     Applications      • Molecular cloning      • Restriction site mapping      • Genotyping      • Southern Blot      • Restriction fragment length polymorphism (RFLP)      • SNP analysis
 
     Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications 
     as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration
     measurements in UV light) the colorless FastDigest Buffer is recommended.
 
<div>     For methylation sensitivity, refer to product specifications.
</div>

Fermentas FD0303 FASTDIGEST ECO32I EA 200 reactions

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