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An E. coli strain that carries the cloned and modified BbsI gene from Bacillus laterosporus (C. Story)
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货品编号: NEB .R3539S
品牌: NEB
品名: BbsI-HF®
规格: 300 units
Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Reaction Conditions 1X CutSmart® Buffer Incubate at 37°C 1X CutSmart® Buffer: 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 μg/ml BSA pH 7.9 @ 25°C Activity in NEBuffers NEBuffer 1.1: 10% NEBuffer 2.1: 10% NEBuffer 3.1: 10% CutSmart® Buffer: 100% Diluent Compatibility Diluent B Storage Temperature -20°C Storage Conditions 10 mM Tris-HCl 300 mM NaCl 1 mM DTT 0.1 mM EDTA 200 μg/ml BSA 50% Glycerol pH 7.4 @ 25°C Heat Inactivation65°C for 20 min Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive
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