Invitrogen.10359016 Bac-to-Bac™ Baculovirus Expression System 1 kit

The Bac-to-Bac® Baculovirus Expression System enables the efficent production of recombinant baculovirus for expression testing in insect cells.

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货品编号：Invitrogen.10359016

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品牌：Invitrogen
所属分类：培养基

<div>
 
</div>     货品编号：  Invitrogen.10359016   
     品牌：  <span style="display: inline !important; float: none; background-color: transparent; color: rgb(0, 0, 0); cursor: text; font-family: Arial,Verdana,sans-serif; font-size: 12px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;">Invitrogen
     品名：  <span style="display: inline !important; float: none; background-color: transparent; color: rgb(0, 0, 0); cursor: text; font-family: Arial,Verdana,sans-serif; font-size: 12px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;">Bac-to-Bac™ Baculovirus Expression System  
     规格：  <span style="display: inline !important; float: none; background-color: transparent; color: rgb(0, 0, 0); cursor: text; font-family: Arial,Verdana,sans-serif; font-size: 12px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;">1 kit
 
     产品描述：
     The Bac-to-Bac® Baculovirus Expression System enables the efficent production of recombinant baculovirus for expression testing in
     insect cells. The Bac-to-Bac® System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than
     homologous recombination in insect cells (Figure 1). This system features:
 
     • Time-saving expression bacmid. With Bac-to-Bac®, the expression cassette of the pFastBac™ vector recombines with the parent 
     bacmid in DH10Bac™ E. coli Competent Cells (included in this system kit) to form an expression bacmid. The bacmid is then transfected 
     into insect cells for production of recombinant baculovirus particles.      • Easy colony screening. The parent bacmid in DH10Bac™ E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon 
     transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of 
     recombinant colonies easy.      • Flexibility. The Bac-to-Bac® Baculovirus Expression System comes with a pFastBac™ 1 vector that offers the strong polyhedrin promoter
     for protein expression and a large multiple cloning site for simplified cloning. Two other vectors are offered separately: pFastBac™ HT vector
     (Cat. No. 10584-027) for production of histidine-tagged recombinant protein and pFastBac™ Dual vector (Cat. No. 10712-024) for expression 
     of two proteins simultaneously.      • Rapid purification of recombinant proteins. The optional pFastBac™ HT vector (Cat. No. 10584-027) produces histidine-tagged recombinant 
     protein. This allows rapid protein purification with nickel-chelating resin.

Invitrogen.10359016 Bac-to-Bac™ Baculovirus Expression System 1 kit

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