zymo.M3011 Dual Media Set(100ml EB&500ml OB) 1 Set

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: 首页生物试剂表观遗传学(专题) DNA修饰的检测定量 zymo.M3011 Dual Media Set(100ml EB&500ml OB) 1 Set

zymo.M3011 Dual Media Set(100ml EB&500ml OB) 1 Set

Simple, reliable method for high level recombinant protein expression in E. coli.

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货品编号：zymo.M3011

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品牌：Zymo
所属分类：DNA修饰的检测定量

货品编号：  zymo.M3011       品牌：  zymo      品名：  Dual Media Set(100ml EB&500ml OB)      规格：  1 Set         产品描述：       Although recombinant protein expression in E. coli has become routine, high level protein expression or overexpression      is not always attainable for every protein. Our research has shown that high level protein expression can be achieved      consistently when two processes, cell expansion and protein expression, are kept separate.     The Dual Media Set™, different from commonly used protein expression procedures using Luria-Bertani (LB) medium or      other specially prepared medium, contains two specially formulated media: Expansion Broth (EB) and Overexpression      Broth (OB). For expansion, E.coli cells are grown in EB which keeps the production of recombinant protein repressed. To      initiate high level protein expression, OB is simply added to the culture. By using the Dual Media Set™, protein      overexpression can be reliably controlled for many recombinant proteins (see Figure 2). In some circumstances, when the      expressed protein is either toxic or insoluble, overexpression may be counter-productive. In such cases, protein production      can be kept at a minimum by adding the inducer IPTG (for lac-based promoters) to cells growing in EB (see Figure 1).