Proteintech 60004-1-Ig，GAPDH Monoclonal Anbody ; Proteintech.60004-1-Ig ; Tested applicaons: ELISA, WB, IF, FC, IP, IHC ;

    Synonyms: GAPDH; G3PD, GAPD, GAPDH, OK/SW cl.12

    Species specificity: Human, Mouse, Rat, Zebrafish, Yeast; other species not tested. 

    Cited species: C. elegans, Cyprinus carpio, hamster, human, mouse, mussel P. viridis, porcine, rat, Yeast, zebrafish 

    Posive WB detected in Mul-cells/ssue, arabidopsis whole plant ssue, corn whole plant ssue, HEK-293 cells, HeLa cells, Raji cells, rice whole plant ssue, soybean whole plant ssue, wheat whole plant ssue, whole Nematode ssue, whole yeast cells, zebrafish ssue 

                                                   FC: N/A

    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylaon of glyceraldehyde-3-phosphate during glycolysis. GAPDH parcipates in nuclear events including transcripon, binding RNA, RNA transportaon, DNA replicaon, DNA repair and apoptosis. Being stably and constuvely expressed at high levels in most ssues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blo+ng. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modificaon such as S-nitrosylaon. This anbody is a mouse monoclonal anbody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/ssues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

    Full name: Glyceraldehyde-3-phosphate dehydrogenase

    Storage: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20 oC.

     

     Western blot analysis of GAPDH in various ssues and cell lines using Proteintech anbody 60004-1-Ig at a diluon of 1:10000.

    IP result of an-GAPDH (60004-1-Ig for IP and Detecon) with HeLa cell lysate.

    Western blot of Hela cell with an- GAPDH (60004-1-Ig) at various diluons.

    Immunohistochemistry of paraffin-embedded human breast cancer slide using 60004-1-Ig( GAPDH Anbody) at diluon of 1:50

    Immunohistochemistry of paraffin-embedded human breast cancer slide using 60004-1-Ig( GAPDH Anbody) at diluon of 1:5

    Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004-1-Ig( GAPDH Anbody) at diluon of 1:50 and Rhodaminelabeled goat an-mouse IgG (red).

     

    1X10^6 HeLa cells were stained with 0.2ug GAPDH anbody (60004-1-Ig, red) and control anbody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat an-Mouse IgG with diluon 1:100.