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Catalog number: 60008-1-Ig
Size: 150 μg/150 μl
Source: Mouse
Isotype: IgM
Synonyms: ACTB; ACTB, Acn, acn, beta, Acn, cytoplasmic 1, B acn, Beta acn, F acn, β acn
Applications:
Tested applicaons: ELISA, WB, IF, FC, IHCCited applicaons: IHC, WB
Species specificity: Human, Mouse, Rat, Zebrafish, Pig, yeast; other species not tested.Cited species: Bovine, C. elegans, chicken, Drosophila, duck, fly, human, mouse, pig, rat, rice, yeast, zebrafishCaculated beta Actin MW: 375aa,42 kDa
Observed beta Actin MW: 42 kDa
Posive WB detected in : Mul-cells/ssue, A549 cells, arabidopsis whole plant ssue, HEK-293 cells, HeLa cells, MCF7 cells, whole Nematode ssue, whole yeast cellsPosive IHC detected in: Human kidney ssue, human brain ssue, human colon ssue, human heart ssue, human lung ssue, human ovary ssue, human placenta ssue, human spleen ssue, human tess ssue
Posive IF detected in : HepG2 cells
Posive FC detected in: HepG2 cells
Recommended diluon: WB: 1:2000-1:20000
IHC: 1:20-1:200
IF: 1:20-1:200
FC: N/ABackground:
Beta acn, also named as ACTB and F-Acn, belongs to the acn family. Acns are highly conserved globular proteins that are involved in various types of cell molity and are ubiquitously expressed in all eukaryoc cells. At least six isoforms of acns are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aorc smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle acn proteins. Most acns consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta acn has been widely used as the internal control in RT-PCR and Western Blo0ng as a 42- kDa protein. This anbody can recognize all the acns.Immunogen information:
Immunogen: Ag0297
GenBank accession number: BC002409
Gene ID (NCBI): 60
Full name: Actin, betaProduct information
Purificaon method: Caprylic acid/ammonium sulfate precipitaon
Storage: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20 oC.
Western blot analysis of Beta-acn in various ssues and cell lines using Proteintech anbody 60008-1-Ig at a diluon of 1:5000. Extra bands were detected in some species with unknown reason.
Western blot of Hela cell with an- Acn-Beta (60008-1-Ig) at various diluons.
Immunofluorescent analysis of HepG2 cells using 60008-1-Ig( beta acn Anbody) at diluon of 1:50 and N/A
Immunohistochemical of paraffin-embedded human kidney using 60008-1-Ig(ACTB anbody) at diluon of 1:100 (under 40x lens)
1X10^6 HepG2 cells were stained with 0.2ug beta acn anbody (60008-1-Ig, red) and control anbody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat An-Mouse IgG(H+L) with diluon 1:1000.