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  • Proteintech 66008-2-Ig，Flagtag Monoclonal Anbody;Proteintech.66008-2-Ig; Tested applicaons: ELISA, WB, IP,
  • Proteintech 66008-2-Ig，Flagtag Monoclonal Anbody;Proteintech.66008-2-Ig; Tested applicaons: ELISA, WB, IP,
  • Proteintech 66008-2-Ig，Flagtag Monoclonal Anbody;Proteintech.66008-2-Ig; Tested applicaons: ELISA, WB, IP,
  • Proteintech 66008-2-Ig，Flagtag Monoclonal Anbody;Proteintech.66008-2-Ig; Tested applicaons: ELISA, WB, IP,

Proteintech 66008-2-Ig，Flagtag Monoclonal Anbody;Proteintech.66008-2-Ig; Tested applicaons: ELISA, WB, IP,

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  •  

        Catalog number: 66008-2-Ig 

        Size: 150 μg/150 μl 

        Source: Mouse 

        Isotype: IgG2b

        Synonyms: DDK tag, DYKDDDDK, FLAG, Flag tag

     

        Applications:

        Tested applicaons: ELISA, WB, IF, IP

        Cited applicaons:  WB 

        Species specificity: Human; other species not tested.
        Cited species: Rat

        Caculated Flag tag MW: -

        Observed Flag tag MW: -

        Posive WB detected in :Recombinant protein, Transfected HEK-293 cells

        Posive IP detected in : Recombinant protein

        Posive IF detected in: Transfected HEK-293 cells

        Recommended diluon:    WB: 1:5000-1:50000  

                                                        IP:  N/A

                                                        IF: 1:20-1:200 

     

     

        Background:

        Flag tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of Flag tag has been opmized for compability with the proteins it is aCached to and unlikely to denature or inacvated them. For its high hydrophilic nature, the Flag tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to anbodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more Flag tags, no maCer it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with Flag tagged fusion protein does not depend on calcium ion. The clone of this monoclonal anbody hybridoma is 1E7B4, which is different from any other vendors.

     

        Immunogen information:

        Immunogen: Pepde 

        GenBank accession number:  /

        Gene ID (NCBI):  /

        Full name: Undefined

     

        Product information 

        Purificaon method: Protein A purificaon

        Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20 oC.

     

      

        IP Result of an-Flag tag (IP:66008-2- Ig, 3ug; Detecon:20543-1-AP 1:2000) with Recombinant protein protein lysate 1280ug. 

     

                     

        Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-2-Ig( Flag tag Anbody) at diluon of 1:1000

     

          

         Immunofluorescent analysis of Transfected HEK-293 cells using 66008-2-Ig( Flag tag Anbody) at diluon of 1:50 and Alexa Fluor 594- congugated AffiniPure Goat An- Mouse IgG(H+L)

     

         

        Recombinant protein were subjected to SDS PAGE followed by western blot with 66008-2-Ig (Flag tag Anbody) at various diluons.