- 商品详情
- 基本信息
- 用户评论0
- 用户咨询0
-
Catalog number: 66002-1-Ig
Size: 213 μg/150 μl
Source: Mouse
Isotype: IgG2a
Synonyms: CFP, eGFP, eYFP, GFP, GFP tag, YFP
Applications:
Tested applicaons: ELISA, WB, IF,IPCited applicaons: IF, IP, WB
Species specificity: Recombinant protein; other species not tested.Cited species: Tag
Caculated GFP tagn MW: 26 kDa
Observed GFP tag MW: /
Posive WB detected in : Recombinant ProteinPosive IP detected in: Transfected HEK-293 cells
Posive IF detected in : Transfected HEK-293 cells
Recommended diluon: WB: 1:2000-1:20000
IP: 1:1000-1:10000
IF: 1:10-1:100
Background:
Protein tags are protein or pepde sequences located either on the C- or Nterminal of the target protein, which facilitates one or several of the following characteriscs: solubility, detecon, purificaon, localizaon and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitaon peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substuons Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shiFed excitaon peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its exncon coefficient(Em). The anbody recognizes the GFP-tag, eGFP tag, eYPF tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.Immunogen information:
Immunogen: Ag2128
GenBank accession number: AVU73901
Gene ID (NCBI): /
Full name: UndefinedProduct information
Purificaon method: Protein A purificaon
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20 oC
WB result of eGFP fusion protein with an-eGFP tag(66002-1-Ig) with GFP fusion protein at varous diluons.
Immunofluorescent analysis of Transfected HEK-293 cells using 66002-1-Ig( GFP tag Anbody) at diluon of 1:25 and Alexa Fluor 488- congugated AffiniPure Goat An- Mouse IgG(H+L)
IP Result of an-GFP tag (IP:66002-1- Ig, 4ug; Detecon:66002-1-Ig 1:2000) with Transfected HEK-293 cells lysate 300ug.