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Catalog number: HRP-66031
Size: 200 μg/100 μl
Source: Mouse
Isotype: IgG2b
Synonyms: TUBA1B; Alpha tubulin ubiquitous, alpha tubulin,a tubulin, HRP Tubulin Alpha, K ALPHA 1, TUBA1B, tubulin, Tubulin Alpha, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b
Applications:
Tested applicaons: ELISA, WB
Species specificity: Human,Mouse,Rat; other species not tested.
Caculated alpha Tubulin MW: 50 kDaObserved alpha Tubulin MW: 52 kDa
Posive WB detected in : HeLa cells
Recommended diluon: WB: 1:2000-1:10000
Background:
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which mulmerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not idencal. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to anmicrobial and anmitoc drugs. This anody recognize Tubulin-Alpha only and it is conjugated with HRP.Note: Do not add Azium (Sodium Azide or Smite) into the diluon buffer. Azium is the HRP inhibitor which decreases the enzymac acvity of HRP.Immunogen information:
Immunogen: Recombinant Protein
GenBank accession number: BC009314
Gene ID (NCBI): 10376
Full name: Tubulin, alpha 1bProduct information
Purificaon method: Protein A Purified
Storage: PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3. Store at -20 oC.
Western blot analysis of alphatubulin in various ssues and cell lines using Proteintech anbody HRP-60031 at a diluon of 1:5000.