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  • Thermo.11960044	gibco DMEM, high glucose, no glutamine	500 mL /瓶

Thermo.11960044 gibco DMEM, high glucose, no glutamine 500 mL /瓶

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         货品编号:  Thermo.11960044 
         品       牌:  Thermo
         品       名:  gibco DMEM, high glucose, no glutamine 
         规       格:  500 mL /瓶

     

         Storage conditions: 2°C to 8°C (protect from light)
         Shipping conditions: Ambient
         Shelf life: 12 months from date of manufacture
         
         产品描述:
         DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. 

         Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell 

         lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the 

         right formulation using the media selector tool.

         This DMEM is modified as follows:With Without 
         • High Glucose • L-glutamine 
         • Phenol Red • Sodium Pyruvate 
         • HEPES

         The complete formulation is available.

     

         Using DMEM
         DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal 

         Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose 

         levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, 

         commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% 

         CO2 environment to maintain physiological pH.