Proteintech HRP-66002，HRP-conjugated GFP tag Monoclonal Antibody;Proteintech.HRP-66002; Tested applicaons: ELISA, WB

    Catalog number: HRP-66002

    Size: 200 μg/100 μl 

    Source: Mouse 

    Isotype: IgG2b

    Synonyms: CFP, eGFP, eYFP, GFP, GFP tag, HRP CFP, HRP eGFP, HRP GFP, HRP YFP, YFP

    Applications:

    Tested applicaons: ELISA, WB

    Species specificity: Recombinant protein, Aequorea victoria; other species not tested.

    Observed GFP tagn  MW:  /

    Positive WB detected in :Recombinant protein

    Recommended diluon:    WB: 1:5000-1:50000  

    Background:

    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYPF tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. This antibody is conjugated with HRP.

    Immunogen information:

    Immunogen: Recombinant Protein

    GenBank accession number: AVU73901

    Gene ID (NCBI):  /

    Product information 

    Purificaon method: Protein A Purified

    Storage: PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3. Store at -20 oC.

    Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66002(GFP tag Atibody) at dilution of 1:10000