thermo.FD2164 Thermo Scientific™ FastDigest KflI 20 reactions

     universal FastDigest Buffer. Isoschizomers: SanDI, Sse1825I.

     Thermo Scientific FastDigest KflI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal 

     FastDigest and FastDigest Green reaction buffers.

     The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer 

     change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat 

     inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow 

     Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, 

     enzymes for downstream applications can be directly added to the FastDigest reaction mix.

     For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion 

     reaction products on gels.

     Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

     Features

     • 100% activity of all FastDigest enzymes in the universal buffer
     • 100% buffer compatibility with downstream applications
     • Complete digestion in 5–15 minutes
     • Direct loading on gels
     • No star activity
     • 176 FastDigest enzymes available

     Applications

     • Molecular cloning
     • Restriction site mapping
     • Genotyping
     • Southern Blot
     • Restriction fragment length polymorphism (RFLP)
     • SNP analysis