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货品编号： R&D.455-MC-010品牌：R&D systems
品名：Recombinant Mouse SCF Protein
规格：10ug
保存：-20°C~ -70 °C
说明书下载：Recombinant Mouse SCF Protein
Purity： >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Endotoxin Level：<0.10 EU per 1 μg of the protein by the LAL method.
Activity： Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura,
T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is typically 2.5-10 ng/mL
Source：E. coli-derived Lys26-Ala189, with an N-terminal Met
Accession #： #Q78ED8
N-terminal Sequence Analysis： MetPredicted Molecular Mass： 18.6 kDa
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Formulation： Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution： Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping： The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage： Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
SDS-PAGE
1 μg/lane of Recombinant Mouse SCF/c-kit was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining,
showing a single band at 19 kDa.
Bioactivity
Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC) stimulates cell proliferation of the TF-1 human erythroleukemic cell line.
The ED50 for this effect is typically 2.5-10 ng/mL.
Background: SCF/c-kit Ligand
Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 - 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72% - 75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).