Sigma T8787-100ML Triton™ X-100

     货品编号：  Sigma.T8787-100ML

     品牌：  Sigma

     品名：  Triton X-100

     General description
     Triton X-100 is a non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins.
     Triton X-100 has no antimicrobial properties.

     It is considered as a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent.

     It does absorb in the ultraviolet region of the spectrum, however, so can interfere with protein quantitation.

     Application
     Triton™ X-100 has been used:
     • To permeabilise cells during Immunofluorescent microscopic studies
     • In immunohistochemistry for staining the Flat-mount retinas
     • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure

     Related Categories
     Antigen-Vaccine Preparation, Apoptosis and Cell Cycle, Biochemicals, Cell Culture, Core Bioreagents, Detergents,  Detergents A to M, 

     Detergents N to Z,  Detergents by Applications,  Diagnostic Applications,  Electrophoresis Chromatography,  Enzymology,  General Reagents, 

     Life  Science Reagents for Nucleic Acid Purification,  Life Science Reagents for Protein Electrophoresis,  Life Science Reagents for Protein Expression

     and Purification,  Life Science Reagents for RNAi,  Life Science Reagents for Western Blotting,  Membrane Protein Solubilization,  Molecular Biology, 
     Non-Ionic Detergents,  Other Apoptosis Related Reagents,  Research Essentials,  分子生物学,  分子生物学试剂,  去垢剂,  生化试剂,  细胞信号转导和神经科学,  细胞生物学

     grade                                   for molecular biology 
     description                         non-ionic 
     form                                     liquid 
     mol wt                                  micellar avg mol wt 80,000 
                                                  average mol wt 625 
     aggregation number          100-155 
     CMC                                     0.2-0.9 mM(20-25°C) 
     transition temp                   cloud point 65 °C 
                                                  pour point ~7 °C 
     solubility                             water: soluble0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow 
     density                                1.06 g/mL at 25 °C(lit.) 
     HLB                                     13.5 
     foreign activity                   DNase and RNase, none detected 

     安全信息