Thermo.11995065 DMEM, high glucose, pyruvate 500ml

     货品编号：  Thermo.11995065

     品牌：  Invitrogen

     品名：  DMEM, high glucose, pyruvate

     规格：  500ml

     产品描述

     DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many

     different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells,

     HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety

     of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

     This DMEM is modified as follows:
     With                                             Without
     • High Glucose                             • HEPES
     • L-glutamine
     • Phenol Red
     • Sodium Pyruvate
     The complete formulation is available.

     Using DMEM
     DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original

     Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is

     often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors.

     Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate

     buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

     Product use
     For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law

     restricts this device to sale by or on the order of a physician. Customers using Gibco® DMEM in a manufacturing process,

     who have a submission with the FDA, may request from us a letter of authorization to reference our Type II Drug Master File (DMF).

     cGMP manufacturing and quality system
     DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA

     as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM

     product made in our Scotland facility (41966-029). This facility is registered with the FDA as a medical device manufacturer and is

     certified to the ISO 13485 standard.