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  • Thermo 22400-089 RPMI 1640 Medium, HEPES 500 mL

Thermo 22400-089 RPMI 1640 Medium, HEPES 500 mL

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         货品编号:  Thermo.22400-089

         品牌:  Thermo

         品名:  RPMI 1640 Medium, HEPES

         规格:  500 mL/瓶

     

         产品描述:

         RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

         Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells,

         including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium

         modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
         This RPMI is modified as follows:


         With                                Without
         • L-glutamine
         • HEPES
         • Phenol Red
         The complete formulation is available.

     

         Using RPMI
         RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations

         of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential

         Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

         RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation,

         commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and

         therefore requires a 5–10% CO2 environment to maintain physiological pH.

     

         Product use
         For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation

         of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a

         letter of authorization from us to reference our Type II Drug Master File (DMF).

     

         cGMP manufacturing and quality system
         RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with

         the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical

         RPMI 1640 product made in our Scotland facility (52400-025). This facility is registered with the FDA as a medical device manufacturer

         and is certified to the ISO 13485 standard.