Invitrogen.10359016 Bac-to-Bac™ Baculovirus Expression System 1 kit

     货品编号：  Invitrogen.10359016   

     品牌：  Invitrogen

     品名：  Bac-to-Bac™ Baculovirus Expression System  

     规格：  1 kit

     产品描述：

     The Bac-to-Bac® Baculovirus Expression System enables the efficent production of recombinant baculovirus for expression testing in

     insect cells. The Bac-to-Bac® System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than

     homologous recombination in insect cells (Figure 1). This system features:

     • Time-saving expression bacmid. With Bac-to-Bac®, the expression cassette of the pFastBac™ vector recombines with the parent

     bacmid in DH10Bac™ E. coli Competent Cells (included in this system kit) to form an expression bacmid. The bacmid is then transfected

     into insect cells for production of recombinant baculovirus particles.
     • Easy colony screening. The parent bacmid in DH10Bac™ E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon

     transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of

     recombinant colonies easy.
     • Flexibility. The Bac-to-Bac® Baculovirus Expression System comes with a pFastBac™ 1 vector that offers the strong polyhedrin promoter

     for protein expression and a large multiple cloning site for simplified cloning. Two other vectors are offered separately: pFastBac™ HT vector

     (Cat. No. 10584-027) for production of histidine-tagged recombinant protein and pFastBac™ Dual vector (Cat. No. 10712-024) for expression

     of two proteins simultaneously.
     • Rapid purification of recombinant proteins. The optional pFastBac™ HT vector (Cat. No. 10584-027) produces histidine-tagged recombinant

     protein. This allows rapid protein purification with nickel-chelating resin.