zymo.M3011 Dual Media Set(100ml EB&500ml OB) 1 Set

 
     货品编号：  zymo.M3011 
     品牌：  zymo
     品名：  Dual Media Set(100ml EB&500ml OB)
     规格：  1 Set
  
     产品描述： 
     Although recombinant protein expression in E. coli has become routine, high level protein expression or overexpression
     is not always attainable for every protein. Our research has shown that high level protein expression can be achieved
     consistently when two processes, cell expansion and protein expression, are kept separate.

     The Dual Media Set™, different from commonly used protein expression procedures using Luria-Bertani (LB) medium or
     other specially prepared medium, contains two specially formulated media: Expansion Broth (EB) and Overexpression
     Broth (OB). For expansion, E.coli cells are grown in EB which keeps the production of recombinant protein repressed. To
     initiate high level protein expression, OB is simply added to the culture. By using the Dual Media Set™, protein
     overexpression can be reliably controlled for many recombinant proteins (see Figure 2). In some circumstances, when the
     expressed protein is either toxic or insoluble, overexpression may be counter-productive. In such cases, protein production
     can be kept at a minimum by adding the inducer IPTG (for lac-based promoters) to cells growing in EB (see Figure 1).