easy-to-follow procedure is based on a nickel-charged His-Affinity Gel (IMAC), innovative protein purification,
and unique Zymo-Spin Column technology. Up to 1 mg of His-tagged protein can be purified in as little as 5
minutes and can be eluted into as little as 100 μl of the provided His-Elution Buffer. The purified protein can be
used directly for enzymatic assays, protein biochemical analyses, SDS PAGE, as well as other protein based
applications. The His-Spin Protein Miniprep has been optimized to yield maximal protein purity indices: a single
protein band is often visualized following Coomassie Blue staining of proteins in SDS-PAGE gel (see figure below).
The straightforward spin-wash-elute protocol dramatically simplifies protein purification and results are obtained in
minutes, not hours!